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Addgene

pBS CRABP1
(Plasmid #19189)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19189 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cellular retinoic acid binding protein 1
  • Alt name
    CRABP1
  • Species
    G. gallus (chicken)
  • GenBank ID
    FG356175
  • Entrez Gene
    CRABP1 (a.k.a. CRABP-I, cCRABP-I)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Probe 1_17, expressed in the outer layer of the PC, strong probe for CRABP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS CRABP1 was a gift from Cliff Tabin (Addgene plasmid # 19189 ; http://n2t.net/addgene:19189 ; RRID:Addgene_19189)

  • For your References section:

    Identification of unique molecular subdomains in the perichondrium and periosteum and their role in regulating gene expression in the underlying chondrocytes. Bandyopadhyay A, Kubilus JK, Crochiere ML, Linsenmayer TF, Tabin CJ. Dev Biol. 2008 Sep 1;321(1):162-74. doi: 10.1016/j.ydbio.2008.06.012. Epub 2008 Jun 16. 10.1016/j.ydbio.2008.06.012 PubMed 18602913
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