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Addgene

pKS235
(Plasmid #191855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS+
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5304
  • Modifications to backbone
    No modifications
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    peel-1 toxic protein for negative selection against extrachromosomal array
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2361
  • Promoter hsp-16.41

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T3 promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS235 was a gift from K Subramaniam (Addgene plasmid # 191855 ; http://n2t.net/addgene:191855 ; RRID:Addgene_191855)

  • For your References section:

    An efficient negative selection marker for Mos1 -mediated single-copy integration in Caenorhabditis elegans. Revathi K, Subramaniam K. MicroPubl Biol. 2022 Oct 20;2022:10.17912/micropub.biology.000647. doi: 10.17912/micropub.biology.000647. eCollection 2022. 10.17912/micropub.biology.000647 PubMed 36338151
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