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Addgene

pProEx-HTa-SOD1 C57A/C146A
(Plasmid #191850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProEx-HTa
  • Backbone size w/o insert (bp) 4779
  • Total vector size (bp) 5244
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    465
  • Mutation
    C57A, C146A
  • Entrez Gene
    SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Hind3 (not destroyed)
  • 5′ sequencing primer M13_pUC_Rev_primer
  • 3′ sequencing primer pBAD_Rev_primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    from pF151 pcDNA3.1(+)SOD1WT (addgene)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pProEx-HTa-SOD1 C57A/C146A was a gift from Nam-Chul Ha (Addgene plasmid # 191850 ; http://n2t.net/addgene:191850 ; RRID:Addgene_191850)

  • For your References section:

    Structural analysis of the overoxidized Cu/Zn-superoxide dismutase in ROS-induced ALS filament formation. Baek Y, Woo TG, Ahn J, Lee D, Kwon Y, Park BJ, Ha NC. Commun Biol. 2022 Oct 12;5(1):1085. doi: 10.1038/s42003-022-04017-0. 10.1038/s42003-022-04017-0 PubMed 36224351
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