pDV052
(Plasmid
#191825)
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Purpose(Empty Backbone) Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDV052
- Backbone size (bp) 5106
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDV052 was a gift from Jim Roberts (Addgene plasmid # 191825 ; http://n2t.net/addgene:191825 ; RRID:Addgene_191825) -
For your References section:
Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains. Tabakh H, Jester BW, Zhao H, Kuestner R, Khuong N, Shanitta C, Takeuchi R, Roberts J. STAR Protoc. 2023 Feb 8;4(1):102087. doi: 10.1016/j.xpro.2023.102087. 10.1016/j.xpro.2023.102087 PubMed 36853691
Map uploaded by the depositor.
