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Addgene

pET-28a(+)-His(6)-AtPCO3(wt)
(Plasmid #191800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtPCO3
  • Species
    A. thaliana (mustard weed)
  • Tag / Fusion Protein
    • 6xHis, Thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a(+)-His(6)-AtPCO3(wt) was a gift from Emily Flashman (Addgene plasmid # 191800 ; http://n2t.net/addgene:191800 ; RRID:Addgene_191800)

  • For your References section:

    The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors. White MD, Kamps JJAG, East S, Taylor Kearney LJ, Flashman E. J Biol Chem. 2018 Jul 27;293(30):11786-11795. doi: 10.1074/jbc.RA118.003496. Epub 2018 May 30. 10.1074/jbc.RA118.003496 PubMed 29848548
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