pET-28a(+)-His(6)-AtPCO2(wt)
(Plasmid
#191799)
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PurposeFull length wt AtPCO2 with Nt-His tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAtPCO2
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SpeciesA. thaliana (mustard weed)
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Tag
/ Fusion Protein
- 6xHis, Thrombin cleavage site (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-28a(+)-His(6)-AtPCO2(wt) was a gift from Emily Flashman (Addgene plasmid # 191799 ; http://n2t.net/addgene:191799 ; RRID:Addgene_191799) -
For your References section:
The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors. White MD, Kamps JJAG, East S, Taylor Kearney LJ, Flashman E. J Biol Chem. 2018 Jul 27;293(30):11786-11795. doi: 10.1074/jbc.RA118.003496. Epub 2018 May 30. 10.1074/jbc.RA118.003496 PubMed 29848548