AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR Sequences (3)

Addgene Sequences:

Full (1)

Depositing Scientist Sequences:

Partial (2)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

Analyze Sequence

GenBank

SnapGene

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> Addgene NGS Result CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGG CCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTGCGGCCTCTA GATAGGTCTTGAAAGGAGTGGGAATTGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGT CCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGG GAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAG TCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGACCGGTGCCACCATGCCTAAAA AGAAAAGAAAGGTGGGTTCTGGTATCGAGAAGAAGAAGAGCTTCGCCAAGGGCATGGGAGTGAAGAGCAC CCTGGTGTCCGGCTCTAAGGTGTACATGACCACATTTGCTGAGGGAAGCGACGCCAGGCTGGAGAAGATC GTGGAGGGCGATAGCATCAGATCCGTGAACGAGGGAGAGGCTTTCAGCGCCGAGATGGCTGACAAGAACG CTGGCTACAAGATCGGAAACGCCAAGTTTTCCCACCCAAAGGGCTACGCCGTGGTGGCTAACAACCCACT GTACACCGGACCAGTGCAGCAGGACATGCTGGGACTGAAGGAGACACTGGAGAAGAGGTACTTCGGCGAG TCCGCCGACGGAAACGATAACATCTGCATCCAGGTCATCCACAACATCCTGGATATCGAGAAGATCCTGG TGGAGTACATCACAAACGTGGTGTACGTGGTGAACAACATCTCCGGCCTGGACAAGGATATCATCGGCTT CGGAAAGTTTTCTACCGTGTACACATACGACGAGTTCAAGGATCCAGAGCACCACCGGGCCGCTTTTAAC AACAACGACAAGCTGATCAACGCCATCAAGGCTCAGTACGACGAGTTCGATAACTTTCTGGATAACCCCA GGCTGGGCTACTTCGGACAGGCTTTCTTTTCTAAGGAGGGCAGAAACTACATCATCAACTACGGAAACGA GTGTTACGACATCCTGGCCCTGCTGAGCGGACTGAGGCACTGGGTGGTGCACAACAACGAGGAGGAGTCT CGGATCAGCCGCACCTGGCTGTACAACCTGGACAAGAACCTGGATAACGAGTACATCTCCACACTGAACT ACCTGTACGACAGGATCACCAACGAGCTGACAAACAGCTTCTCCAAGAACTCTGCCGCTAACGTGAACTA CATCGCTGAGACCCTGGGCATCAACCCAGCTGAGTTCGCTGAGCAGTACTTCAGATTTTCCATCATGAAG GAGCAGAAGAACCTGGGCTTCAACATCACAAAGCTGAGAGAAGTGATGCTGGACAGAAAGGATATGTCCG AGATCAGGAAGAACCACAAGGTGTTCGATTCTATCAGAACCAAGGTGTACACAATGATGGACTTTGTGAT CTACAGGTACTACATCGAGGAGGATGCCAAGGTGGCCGCTGCCAACAAGAGCCTGCCCGACAACGAGAAG TCTCTGAGCGAGAAGGATATCTTCGTGATCAACCTGAGAGGCTCCTTTAACGACGATCAGAAGGACGCTC TGTACTACGATGAGGCCAACAGGATCTGGAGAAAGCTGGAGAACATCATGCACAACATCAAGGAGTTCCG GGGAAACAAGACCCGCGAGTACAAGAAGAAGGACGCTCCAAGGCTGCCTAGGATCCTGCCTGCTGGAAGG GACGTGAGCGCCTTCAGCAAGCTGATGTACGCCCTGACAATGTTTCTGGACGGAAAGGAGATCAACGATC TGCTGACCACACTGATCAACAAGTTCGACAACATCCAGTCTTTTCTGAAAGTGATGCCTCTGATCGGCGT GAACGCTAAGTTCGTGGAGGAGTACGCCTTCTTTAAGGACAGCGCCAAGATCGCTGATGAGCTGCGGCTG ATCAAGTCCTTTGCCAGGATGGGAGAGCCAATCGCTGACGCTAGGAGAGCTATGTACATCGATGCCATCC GGATCCTGGGAACCAACCTGTCTTACGACGAGCTGAAGGCTCTGGCCGACACCTTCAGCCTGGATGAGAA CGGCAACAAGCTGAAGAAGGGCAAGCACGGAATGCGCAACTTCATCATCAACAACGTGATCAGCAACAAG CGGTTTCACTACCTGATCAGATACGGCGACCCAGCTCACCTGCACGAGATCGCTAAGAACGAGGCCGTGG TGAAGTTCGTGCTGGGACGGATCGCCGATATCCAGAAGAAGCAGGGCCAGAACGGAAAGAACCAGATCGA CCGCTACTACGAGACCTGCATCGGCAAGGATAAGGGAAAGTCCGTGTCTGAGAAGGTGGACGCTCTGACC AAGATCATCACAGGCATGAACTACGACCAGTTCGATAAGAAGAGATCTGTGATCGAGGACACCGGAAGGG AGAACGCCGAGAGAGAGAAGTTTAAGAAGATCATCAGCCTGTACCTGACAGTGATCTACCACATCCTGAA GAACATCGTGAACATCAACGCTAGATACGTGATCGGCTTCCACTGCGTGGAGCGCGATGCCCAGCTGTAC AAGGAGAAGGGATACGACATCAACCTGAAGAAGCTGGAGGAGAAGGGCTTTAGCTCCGTGACCAAGCTGT GCGCTGGAATCGACGAGACAGCCCCCGACAAGAGGAAGGATGTGGAGAAGGAGATGGCCGAGAGAGCTAA GGAGAGCATCGACTCCCTGGAGTCTGCTAACCCTAAGCTGTACGCCAACTACATCAAGTACTCCGATGAG AAGAAGGCCGAGGAGTTCACCAGGCAGATCAACAGAGAGAAGGCCAAGACCGCTCTGAACGCCTACCTGA GGAACACAAAGTGGAACGTGATCATCCGGGAGGACCTGCTGCGCATCGATAACAAGACCTGTACACTGTT CCGGAACAAGGCTGTGCACCTGGAGGTGGCTCGCTACGTGCACGCCTACATCAACGACATCGCCGAGGTG AACTCCTACTTTCAGCTGTACCACTACATCATGCAGAGGATCATCATGAACGAGAGATACGAGAAGTCTA GCGGCAAGGTGTCTGAGTACTTCGACGCCGTGAACGATGAGAAGAAGTACAACGATAGACTGCTGAAGCT GCTGTGCGTGCCTTTCGGATACTGTATCCCACGGTTTAAGAACCTGAGCATCGAGGCCCTGTTCGACCGC AACGAGGCTGCCAAGTTTGATAAGGAGAAGAAGAAGGTGAGCGGCAACTCCGATTACAAAGACGATGACG ATAAGCCCAAGAAGAAGAGGAAAGTCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCC GTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGC ATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGA AGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGGATGGGCTGGCCTTTTGCTCAGCTAGCGA GGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATT AATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGT TTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTT CTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCGCAAGTAAACCCCTACCAACTGGTCGGGGTTTG AAACGTGGGTGCCGTGGCTGTCACACTTGCTCGCCAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAAC CAGCATCCCGGCCGCTGACCACACCTGCCACAAGTAAACCCCTACCAACTGGTCGGGGTTTGAAACTTTT TTTGAATTCTGATGCGGTATTTTCTCCTTACGCTGATGCGGTGGCGGCCGCAGGAACCCCTAGTGATGGA GTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCG GGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATT TTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGC GGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGC CCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCG GGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGAT GGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTA ATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGG GATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAAC AAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCC AGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAG ACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGA CGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAG GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTA TCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGC TGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAG CGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTA TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGA ATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATG CAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGA ATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACT ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGC GTGGAAGCCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACAC GACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG CATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTA AAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCA CTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGC TGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTT TTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGG CCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCT GCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGT CGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT ACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGC AGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCG GGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT

Partial Sequences from Depositor (2)

Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.

Analyze Sequence

GenBank

SnapGene

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> pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA taggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtcc ccgagaagttggggggaggggtcggcaattgatccggtgcctagagaaggtggcgcggggtaaactggga aagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtc gccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggaccggtGCCACCATGCCTAAAAAG AAAAGAAAGGTGGGTTCTGGTATCGAGAAGAAGAAGAGCTTCGCCAAGGGCATGGGAGTGAAGAGCACCC TGGTGTCCGGCTCTAAGGTGTACATGACCACATTTGCTGAGGGAAGCGACGCCAGGCTGGAGAAGATCGT GGAGGGCGATAGCATCAGATCCGTGAACGAGGGAGAGGCTTTCAGCGCCGAGATGGCTGACAAGAACGCT GGCTACAAGATCGGAAACGCCAAGTTTTCCCACCCAAAGGGCTACGCCGTGGTGGCTAACAACCCACTGT ACACCGGACCAGTGCAGCAGGACATGCTGGGACTGAAGGAGACACTGGAGAAGAGGTACTTCGGCGAGTC CGCCGACGGAAACGATAACATCTGCATCCAGGTCATCCACAACATCCTGGATATCGAGAAGATCCTGGtg GAGTACATCACAAACGtgGtgTACGtgGTGAACAACATCTCCGGCCTGGACAAGGATATCATCGGCTTCG GAAAGTTTTCTACCGTGTACACATACGACGAGTTCAAGGATCCAGAGCACCACCGGGCCGCTTTTAACAA CAACGACAAGCTGATCAACGCCATCAAGGCTCAGTACGACGAGTTCGATAACTTTCTGGATAACCCCAGG CTGGGCTACTTCGGACAGGCTTTCTTTTCTAAGGAGGGCAGAAACTACATCATCAACTACGGAAACGAGT GTTACGACATCCTGGCCCTGCTGAGCGGACTGAGGCACTGGGTGGTGCACAACAACGAGGAGGAGTCTCG GATCAGCCGCACCTGGCTGTACAACCTGGACAAGAACCTGGATAACGAGTACATCTCCACACTGAACTAC CTGTACGACAGGATCACCAACGAGCTGACAAACAGCTTCTCCAAGAACTCTGCCGCTAACGTGAACTACA TCGCTGAGACCCTGGGCATCAACCCAGCTGAGTTCGCTGAGCAGTACTTCAGATTTTCCATCATGAAGGA GCAGAAGAACCTGGGCTTCAACATCACAAAGCTGAGAGAAGTGATGCTGGACAGAAAGGATATGTCCGAG ATCAGGAAGAACCACAAGGTGTTCGATTCTATCAGAACCAAGGTGTACACAATGATGGACTTTGTGATCT ACAGGTACTACATCGAGGAGGATGCCAAGGTGGCCGCTGCCAACAAGAGCCTGCCCGACAACGAGAAGTC TCTGAGCGAGAAGGATATCTTCGTGATCAACCTGAGAGGCTCCTTTAACGACGATCAGAAGGACGCTCTG TACTACGATGAGGCCAACAGGATCTGGAGAAAGCTGGAGAACATCATGCACAACATCAAGGAGTTCCGGG GAAACAAGACCCGCGAGTACAAGAAGAAGGACGCTCCAAGGCTGCCTAGGATCCTGCCTGCTGGAAGGGA CGTGAGCGCCTTCAGCAAGCTGATGTACGCCCTGACAATGTTTCTGGACGGAAAGGAGATCAACGATCTG CTGACCACACTGATCAACAAGTTCGACAACATCCAGTCTTTTCTGAAAGTGATGCCTCTGATCGGCGTGA ACGCTAAGTTCGTGGAGGAGTACGCCTTCTTTAAGGACAGCGCCAAGATCGCTGATGAGCTGCGGCTGAT CAAGTCCTTTGCCAGGATGGGAGAGCCAATCGCTGACGCTAGGAGAGCTATGTACATCGATGCCATCCGG ATCCTGGGAACCAACCTGTCTTACGACGAGCTGAAGGCTCTGGCCGACACCTTCAGCCTGGATGAGAACG GCAACAAGCTGAAGAAGGGCAAGCACGGAATGCGCAACTTCATCATCAACAACGTGATCAGCAACAAGCG GTTTCACTACCTGATCAGATACGGCGACCCAGCTCACCTGCACGAGATCGCTAAGAACGAGGCCGTGGTG AAGTTCGTGCTGGGACGGATCGCCGATATCCAGAAGAAGCAGGGCCAGAACGGAAAGAACCAGATCGACC GCTACTACGAGACCTGCATCGGCAAGGATAAGGGAAAGTCCGTGTCTGAGAAGGTGGACGCTCTGACCAA GATCATCACAGGCATGAACTACGACCAGTTCGATAAGAAGAGATCTGTGATCGAGGACACCGGAAGGGAG AACGCCGAGAGAGAGAAGTTTAAGAAGATCATCAGCCTGTACCTGACAGTGATCTACCACATCCTGAAGA ACATCGTGAACATCAACGCTAGATACGTGATCGGCTTCCACTGCGTGGAGCGCGATGCCCAGCTGTACAA GGAGAAGGGATACGACATCAACCTGAAGAAGCTGGAGGAGAAGGGCTTTAGCTCCGTGACCAAGCTGTGC GCTGGAATCGACGAGACAGCCCCCGACAAGAGGAAGGATGTGGAGAAGGAGATGGCCGAGAGAGCTAAGG AGAGCATCGACTCCCTGGAGTCTGCTAACCCTAAGCTGTACGCCAACTACATCAAGTACTCCGATGAGAA GAAGGCCGAGGAGTTCACCAGGCAGATCAACAGAGAGAAGGCCAAGACCGCTCTGAACGCCTACCTGAGG AACACAAAGTGGAACGTGATCATCCGGGAGGACCTGCTGCGCATCGATAACAAGACCTGTACACTGTTCC GGAACAAGGCTGTGCACCTGGAGGTGGCTCGCTACGTGCACGCCTACATCAACGACATCGCCGAGGTGAA CTCCTACTTTCAGCTGTACCACTACATCATGCAGAGGATCATCATGAACGAGAGATACGAGAAGTCTAGC GGCAAGGTGTCTGAGTACTTCGACGCCGTGAACGATGAGAAGAAGTACAACGATAGACTGCTGAAGCTGC TGTGCGTGCCTTTCGGATACTGTATCCCACGGTTTAAGAACCTGAGCATCGAGGCCCTGTTCGACCGCAA CGAGGCTGCCAAGTTTGATAAGGAGAAGAAGAAGGTGAGCGGCAACTCCGATTACAAAGACGATGACGAT AAGcccaagaagaagaggAAAGTCctgtgccttctagttgccagccatctgttgtttgcccctcccccgt gccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcat tgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaag acaatagcaggcatgctggggatgcggtgggctctatgg
Analyze Sequence

GenBank

SnapGene

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> pU6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaa ttaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggta gtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgat ttcttggctttatatatcttgtggaaaggacgaaacaccgcaagtaaacccctaccaactggtcggggtt tgaaacgtgggtgccgtggctgtcacacttgctcgccaagtaaacccctaccaactggtcggggtttgaa accagcatcccggccgctgaccacacctgccacaagtaaacccctaccaactggtcggggtttgaaac

AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR Sequences (3)

Full Sequences from Addgene (1)

Partial Sequences from Depositor (2)

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