pFA6a-TRP1-pCUP1-2xphlO
(Plasmid #191794)

• Purpose: Yeast gene induction with copper promoter
• Depositing Lab: Dean Dawson
• Publication: Dawson Lab Plasmids (unpublished)

Backbone

• Vector backbone: pFA6a
• Total vector size (bp): 3960
• Vector type: Yeast genomic targeting
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRP1 pCUP1 with 2 phlO sites
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1479

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pFA6a-TRP1-pCUP1-2xphlO.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University.

New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands
Shigehito Ikushima and Jef D. Boeke
ACS Synthetic Biology 2017 6 (3), 497-506
DOI: 10.1021/acssynbio.6b00205