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Addgene

EC59103
(Plasmid #191785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 4375
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtUbi10::Cas12a+ 8 introns-T-E9
  • Species
    Lachnospiraceae bacterium
  • Insert Size (bp)
    6637
  • Mutation
    D156R

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Made by T Lawrenson

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC59103 was a gift from Wendy Harwood (Addgene plasmid # 191785 ; http://n2t.net/addgene:191785 ; RRID:Addgene_191785)

  • For your References section:

    Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems. Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P. Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16. 10.1007/978-1-0716-3131-7_16 PubMed 36995631
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