pICSL1P6_crU626
(Plasmid
#191781)
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PurposeCas12a guide expression in dicots
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
- Backbone size w/o insert (bp) 4372
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
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Alt namegRNA to be added by user
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SpeciesLachnospiraceae bacterium
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Insert Size (bp)1605
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMade by Mark Youles
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
User inserts own guide. Level 1 position 6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICSL1P6_crU626 was a gift from Wendy Harwood (Addgene plasmid # 191781 ; http://n2t.net/addgene:191781 ; RRID:Addgene_191781) -
For your References section:
Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems. Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P. Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16. 10.1007/978-1-0716-3131-7_16 PubMed 36995631