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pICSL1P3_crU626
(Plasmid #191778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 4372
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
  • Alt name
    gRNA to be added by user
  • Species
    Lachnospiraceae bacterium
  • Insert Size (bp)
    1605

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Made by Mark Youles

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

User inserts own guide. Level 1 position 3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICSL1P3_crU626 was a gift from Wendy Harwood (Addgene plasmid # 191778 ; http://n2t.net/addgene:191778 ; RRID:Addgene_191778)

  • For your References section:

    Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems. Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P. Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16. 10.1007/978-1-0716-3131-7_16 PubMed 36995631
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