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Addgene

EC64422
(Plasmid #191768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 4897
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Alt name
    gRNA to be added by user
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    9419

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

User inserts own guides for CRISPR in Medicago truncatula

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC64422 was a gift from Wendy Harwood (Addgene plasmid # 191768 ; http://n2t.net/addgene:191768 ; RRID:Addgene_191768)

  • For your References section:

    Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method. Lawrenson T, Atkinson N, Forner M, Harwood W. Methods Mol Biol. 2023;2653:221-252. doi: 10.1007/978-1-0716-3131-7_15. 10.1007/978-1-0716-3131-7_15 PubMed 36995630
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