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p80-Katanin-mNeonGreen
(Plasmid #191762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    IRESpuro2
  • Backbone size w/o insert (bp) 5489
  • Total vector size (bp) 7777
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p80-Katanin-mNeonGreen
  • Alt name
    katnb1
  • Species
    H. sapiens (human)
  • Entrez Gene
    KATNB1 (a.k.a. KAT, LIS6)
  • Promoter CMV
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer TACGGTGGGAGGTCTAT
  • 3′ sequencing primer CATGCCCGCTTTTGAGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p80-Katanin-mNeonGreen was a gift from Daniel Gerlich (Addgene plasmid # 191762 ; http://n2t.net/addgene:191762 ; RRID:Addgene_191762)

  • For your References section:

    A mitotic chromatin phase transition prevents perforation by microtubules. Schneider MWG, Gibson BA, Otsuka S, Spicer MFD, Petrovic M, Blaukopf C, Langer CCH, Batty P, Nagaraju T, Doolittle LK, Rosen MK, Gerlich DW. Nature. 2022 Sep;609(7925):183-190. doi: 10.1038/s41586-022-05027-y. Epub 2022 Aug 3. 10.1038/s41586-022-05027-y PubMed 35922507
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