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pcDNA3.1_mGFP-FGFR3-K650E
(Plasmid #191757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5529
  • Total vector size (bp) 8724
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mGFP-FGFR3IIIc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3195
  • Mutation
    c.1948A>G (p.K650E) + silent mutation c.1938C>T generated on purpose
  • GenBank ID
    NM_000142.5
  • Tag / Fusion Protein
    • mGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer pcDNA3.1_R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_mGFP-FGFR3-K650E was a gift from Irene Tiemann-Boege (Addgene plasmid # 191757 ; http://n2t.net/addgene:191757 ; RRID:Addgene_191757)

  • For your References section:

    Measurement of FGFR3 signaling at the cell membrane via total internal reflection fluorescence microscopy to compare the activation of FGFR3 mutants. Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, Tiemann-Boege I. J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832. Epub 2022 Dec 27. 10.1016/j.jbc.2022.102832 PubMed 36581204
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