pcDNA3.1_mGFP-FGFR3-G380R
(Plasmid #191755)

• Purpose: Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)
• Depositing Lab: Irene Tiemann-Boege
• Publication: Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832. Epub 2022 Dec 27.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5529
• Total vector size (bp): 8724
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mGFP-FGFR3IIIc
• Species: H. sapiens (human)
• Insert Size (bp): 3195
• Mutation: c.1138G>A (G380R) + c.1131C>T (silent mutation generated on purpose)
• GenBank ID: NM_000142.5
• Tag / Fusion Protein:
  • mGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: pcDNA3.1_R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry