csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
(Plasmid #191751)

• Purpose: Microtubule marker together with nuclear envelope marker driven by bidirectional promoter
• Depositing Lab: Jay C Dunlap
• Publication: Wang et al Fungal Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763.

Backbone

• Vector backbone: pRS426
• Total vector size (bp): 13454
• Vector type: Bacterial Expression
• Selectable markers: Cyclosporin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NCU04288
• Alt name: son-1
• Species: Neurospora crassa
• Insert Size (bp): 3257
• Entrez Gene: NCU04288 (a.k.a. NCU04288)
• Tags / Fusion Proteins:
  • iRFP670 (C terminal on insert)
  • Gdp1 promoter (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: bml
• Alt name: NCU04054
• Alt name: tubulin beta chain
• Species: Neurospora crassa
• Insert Size (bp): 3085
• Entrez Gene: Bml (a.k.a. NCU04054, Ben, mbic, tub-2)
• Tags / Fusion Proteins:
  • mTagBFP2 (C terminal on insert)
  • ToxA promoter (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pRS426_iRFP670_son-1_GpdToxA_bml_mTagBFP2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry