csr-1_hH1_mEos3.1_V5
(Plasmid
#191750)
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PurposePhotoconvertible fluorescent nuclear reporter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 10614
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Vector typeBacterial Expression
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Selectable markersCyclosporin A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehH1
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Alt nameNCU06863
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SpeciesNeurospora crassa
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Insert Size (bp)2904
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GenBank ID
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Entrez GenehH1 (a.k.a. NCU06863)
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Tags
/ Fusion Proteins
- mEos3.1 (C terminal on insert)
- ccg-1 promoter (N terminal on insert)
- V5 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
csr-1_hH1_mEos3.1_V5 was a gift from Jay C Dunlap (Addgene plasmid # 191750 ; http://n2t.net/addgene:191750 ; RRID:Addgene_191750) -
For your References section:
Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa. Wang Z, Bartholomai BM, Loros JJ, Dunlap JC. Fungal Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763. 10.1016/j.fgb.2022.103763 PubMed 36481248