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Addgene

PL-452 N-Flag-4C
(Plasmid #19175)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PL-452
  • Backbone size w/o insert (bp) 4823
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG tetracysteine tag
  • Alt name
    Flag-4C-PL-452
  • Insert Size (bp)
    137

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Roger Tsien

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PL-452 N-Flag-4C was a gift from Patrik Verstreken (Addgene plasmid # 19175 ; http://n2t.net/addgene:19175 ; RRID:Addgene_19175)

  • For your References section:

    Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation. Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P. Nucleic Acids Res. 2008 Aug 1. ():. 10.1093/nar/gkn486 PubMed 18676454
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