csr-1_son-1_iRFP670
(Plasmid
#191749)
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PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 10967
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Vector typeBacterial Expression
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Selectable markersCyclosporin A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNCU04288
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Alt nameson-1
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SpeciesNeurospora crassa
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Insert Size (bp)3257
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Entrez GeneNCU04288 (a.k.a. NCU04288)
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Tags
/ Fusion Proteins
- iRFP670 (C terminal on insert)
- NCU04502 promoter (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
csr-1_son-1_iRFP670 was a gift from Jay C Dunlap (Addgene plasmid # 191749 ; http://n2t.net/addgene:191749 ; RRID:Addgene_191749) -
For your References section:
Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa. Wang Z, Bartholomai BM, Loros JJ, Dunlap JC. Fungal Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763. 10.1016/j.fgb.2022.103763 PubMed 36481248
Map uploaded by the depositor.
