pGEX-6P--Dr-aCateninFL
(Plasmid
#191740)
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PurposeExpresses FL D rerio catenin in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTNNA1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)2721
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GenBank IDBC155815.1
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Entrez Genectnna1 (a.k.a. ctnna)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX-5'
- 3′ sequencing primer pGEX-3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA obtained from Open Biosystems.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P--Dr-aCateninFL was a gift from Tina Izard (Addgene plasmid # 191740 ; http://n2t.net/addgene:191740 ; RRID:Addgene_191740) -
For your References section:
The nematode alpha-catenin ortholog, HMP1, has an extended alpha-helix when bound to actin filaments. Rangarajan ES, Smith EW, Izard T. J Biol Chem. 2023 Feb;299(2):102817. doi: 10.1016/j.jbc.2022.102817. Epub 2022 Dec 17. 10.1016/j.jbc.2022.102817 PubMed 36539037