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pICH-chl-roGFP2-GPXC71S
(Plasmid #191703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH86988
  • Backbone manufacturer
    Golden Gate Addgene number #48076
  • Backbone size w/o insert (bp) 9078
  • Total vector size (bp) 9983
  • Modifications to backbone
    Added in during cloning: The level 0 chloroplast transiit peptide coding sequence from pICH78133 (Addgene #50292).
  • Vector type
    Plant Expression ; Binary Agrobacterium Ti plasmid
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chl-redox(ro)GFP2-GPXC71S
  • Species
    Synthetic
  • Insert Size (bp)
    1497
  • Mutation
    Peroxidatic Cysteine 71 replaced with Serine
  • Promoter CaMV 35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsa1 (destroyed during cloning)
  • 3′ cloning site Bsa1 (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GPXC71S version was synthesised de novo. Please visit https://doi.org/10.1101/2024.01.18.576236 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICH-chl-roGFP2-GPXC71S was a gift from Philip Mullineaux (Addgene plasmid # 191703 ; http://n2t.net/addgene:191703 ; RRID:Addgene_191703)

  • For your References section:

    A novel glutathione peroxidase-based biosensor disentangles differential subcellular accumulation of H2O2 and lipid hydroperoxides. Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, Mullineaux PM. bioRxiv 2024.01.18.576236 10.1101/2024.01.18.576236
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