pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
(Plasmid #191676)

• Purpose: transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A
• Depositing Lab: Veerle Baekelandt
• Publication: transfer plasmid for lentiviral vector production expressing Hs ATP10B and Hs TMEM30A (unpublished)

Backbone

• Vector backbone: lentiviral transferplasmid
• Backbone manufacturer: Didier Trono
• Backbone size w/o insert (bp): 10215
• Total vector size (bp): 15808
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATP10B
• Species: H. sapiens (human)
• Mutation: D433N
• Entrez Gene: ATP10B (a.k.a. ATPVB)

Gene/Insert 2

• Gene/Insert name: TMEM30A
• Species: H. sapiens (human)
• GenBank ID: NM_018247.4
• Entrez Gene: TMEM30A (a.k.a. C6orf67, CDC50A)
• Tags / Fusion Proteins:
  • 6x HIS (N terminal on insert)
  • flag (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry