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Addgene

pCAG-NCad-B1
(Plasmid #191667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS1
  • Backbone size w/o insert (bp) 4837
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-cadherin
  • Alt name
    NCad
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3614
  • Promoter Chicken β-actin promoter
  • Tags / Fusion Proteins
    • ddFP-B1
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTTCTGGCGTGTGACCGG
  • 3′ sequencing primer GCTTCATGATGTCCCCAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-NCad-B1 was a gift from Takeharu Nagai (Addgene plasmid # 191667 ; http://n2t.net/addgene:191667 ; RRID:Addgene_191667)

  • For your References section:

    Development of intensiometric indicators for visualizing N-cadherin interaction across cells. Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, Matsuda T. Commun Biol. 2022 Oct 7;5(1):1065. doi: 10.1038/s42003-022-04023-2. 10.1038/s42003-022-04023-2 PubMed 36207396
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