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pCRISPomyces-AsCas12j-2
(Plasmid #191655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPomyces-2
  • Backbone size w/o insert (bp) 6888
  • Total vector size (bp) 9159
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AsCas12j-2
  • Alt name
    CasΦ-2
  • Alt name
    Cas12j2
  • Species
    Acidaminococcus sp.
  • Insert Size (bp)
    2274
  • Promoter rpsL(XC)-BbsI

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccgtgagctcgagtagacgacggagacgtaatgccc
  • 3′ sequencing primer ttttgcggggaacgcgtagatctgaattctcagctggtc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-AsCas12j-2 was a gift from Fong Tian Wong (Addgene plasmid # 191655 ; http://n2t.net/addgene:191655 ; RRID:Addgene_191655)

  • For your References section:

    Application of Cas12j for Streptomyces editing and cluster activation. Tan LL, Heng E, Zulkarnain N, Leong CY, Ng V, Yang LK, San Seow DC, Peh G, Lim YH, Lokanand K, Kanagasundaram YH, Ng SB, Wong FT. bioRxiv 2021.10.28.465406 10.1101/2021.10.28.465406
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