Skip to main content
Addgene

pLenti-Sa-gRNA-puro
(Plasmid #191652)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti-gRNA-puro (Addgene #180426)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S. aureus gRNAscr
  • Alt name
    Universal mammalian negative control (scrambled) gRNA for S. aureus
  • gRNA/shRNA sequence
    GCACTACCAGAGCTAACTCA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    467
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer U6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Sa-gRNA-puro was a gift from Moshe Szyf (Addgene plasmid # 191652 ; http://n2t.net/addgene:191652 ; RRID:Addgene_191652)

  • For your References section:

    The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity. Sapozhnikov DM, Szyf M. Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.1038/s41598-023-29332-2. 10.1038/s41598-023-29332-2 PubMed 36759683
Related items: