pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
(Plasmid
#191650)
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PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXD69m2
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCymR
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SpeciesPseudomonas putida F1
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GenBank IDAAB62296.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12) was a gift from Emily Balskus (Addgene plasmid # 191650 ; http://n2t.net/addgene:191650 ; RRID:Addgene_191650) -
For your References section:
Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators. Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP. Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. 10.1038/s41467-022-33576-3 PubMed 36494336