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Addgene

pRSpuro shID1#6
(Plasmid #19165)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroSuper-Puro
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    small hairpin RNA against inhibitor of DNA binding 1
  • Alt name
    ID
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    ID1 (a.k.a. ID, bHLHb24)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for sequence details

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSpuro shID1#6 was a gift from Joan Massague (Addgene plasmid # 19165 ; http://n2t.net/addgene:19165 ; RRID:Addgene_19165)

  • For your References section:

    ID genes mediate tumor reinitiation during breast cancer lung metastasis. Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova K, Gerald WL, Brogi E, Benezra R, Massague J. Proc Natl Acad Sci U S A. 2007 Dec 4. 104(49):19506-11. 10.1073/pnas.0709185104 PubMed 18048329
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