pRShygro shID3#1
(Plasmid
#19164)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 19164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRetroSuper-Hygro
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesmall hairpin RNA against inhibitor of DNA binding 3
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Alt nameHEIR-1
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SpeciesH. sapiens (human)
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Insert Size (bp)64
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Entrez GeneID3 (a.k.a. HEIR-1, bHLHb25)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for sequence details
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRShygro shID3#1 was a gift from Joan Massague (Addgene plasmid # 19164 ; http://n2t.net/addgene:19164 ; RRID:Addgene_19164) -
For your References section:
ID genes mediate tumor reinitiation during breast cancer lung metastasis. Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova K, Gerald WL, Brogi E, Benezra R, Massague J. Proc Natl Acad Sci U S A. 2007 Dec 4. 104(49):19506-11. 10.1073/pnas.0709185104 PubMed 18048329
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/75/75/5bc903e8-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)