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Addgene

pXD70Tet(DadRmt4)-DadR(linker)
(Plasmid #191639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXD69m1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. lenta: Tetracycline 20 μg/mL
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DadR
  • Species
    Eggerthella lenta
  • Mutation
    Linker region sequence shuffled

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXD70Tet(DadRmt4)-DadR(linker) was a gift from Emily Balskus (Addgene plasmid # 191639 ; http://n2t.net/addgene:191639 ; RRID:Addgene_191639)

  • For your References section:

    Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators. Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP. Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. 10.1038/s41467-022-33576-3 PubMed 36494336
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