pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
(Plasmid
#191631)
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PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXD69m2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDopamine dehydroxylase (R506S mutation, inactive form)
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SpeciesEggerthella lenta
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2) was a gift from Emily Balskus (Addgene plasmid # 191631 ; http://n2t.net/addgene:191631 ; RRID:Addgene_191631) -
For your References section:
Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators. Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP. Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. 10.1038/s41467-022-33576-3 PubMed 36494336