pRSpuro shID3#1
(Plasmid #19163)

• Depositing Lab: Joan Massague
• Publication: Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 104(49):19506-11.

Backbone

• Vector backbone: pRetroSuper-Puro
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: small hairpin RNA against inhibitor of DNA binding 3
• Alt name: HEIR-1
• Species: H. sapiens (human)
• Insert Size (bp): 64
• Entrez Gene: ID3 (a.k.a. HEIR-1, bHLHb25)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Supplemental Documents:
  • Cloning and oligo information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See authors map and notes for more details on cloning