pCMV5 TBRI-HA with various GS domain mutations
(Plasmid #19162)

• Depositing Lab: Joan Massague
• Publication: Joan Massague lab plasmids (unpublished)

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4657
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: transforming growth factor, beta receptor 1
• Alt name: TGFB1
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Mutation: T204D (GS domain mutation)
• Entrez Gene: TGFBR1 (a.k.a. AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Weiser
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author map for cloning details.