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Addgene

E1_pENTR_L1-L4_NG_G1397-DddtoxA-N
(Plasmid #191586)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191586 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PINII
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multisite Gateway entry vector to express one hand of pTALECD-UGI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E1_pENTR_L1-L4_NG_G1397-DddtoxA-N was a gift from Shin-ichi Arimura (Addgene plasmid # 191586 ; http://n2t.net/addgene:191586 ; RRID:Addgene_191586)
  • For your References section:

    Targeted base editing in the plastid genome of Arabidopsis thaliana. Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, Arimura SI. Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1. 10.1038/s41477-021-00954-6 PubMed 34211131