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Purpose(Empty Backbone) Expresses an shRNA and mTagBFP2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePLKO.1
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Backbone manufacturerTRC
- Backbone size (bp) 8952
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Modifications to backbonehPGK1 promoter express mTagBFP2 instead of PuroR
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Vector typeMammalian Expression, Lentiviral, RNAi
- Promoter U6 for shRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer U6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.19.594864 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO.1 - TRC mTagBFP2 was a gift from Timothy Ryan (Addgene plasmid # 191566 ; http://n2t.net/addgene:191566 ; RRID:Addgene_191566) -
For your References section:
Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits. Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, Kaplitt MG, De Camilli P, Ryan TA. Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. Epub 2024 Aug 21. 10.1126/sciadv.adn6016 PubMed 39167658
Map uploaded by the depositor.
