HI.fate.UB
(Plasmid
#191556)
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PurposeMonitor HIV-1 fate
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNL4-3.HSA.R-E-
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1 incomplete genome
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SpeciesOther
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Mutationenv-deleted + vpr mutation(s)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original backbone plasmid from NIH AIDS Reagent Program
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HI.fate.UB was a gift from Alon Herschhorn & Joseph Sodroski (Addgene plasmid # 191556 ; http://n2t.net/addgene:191556 ; RRID:Addgene_191556) -
For your References section:
Intra- and extra-cellular environments contribute to the fate of HIV-1 infection. Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, Achuthan V, Engelman AN, Sodroski J, Herschhorn A. Cell Rep. 2021 Aug 31;36(9):109622. doi: 10.1016/j.celrep.2021.109622. 10.1016/j.celrep.2021.109622 PubMed 34469717