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Addgene

HI.fate.SFFV
(Plasmid #191554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNL4-3.HSA.R-E-
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 incomplete genome
  • Species
    Other
  • Mutation
    env-deleted + vpr mutation(s)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original backbone plasmid from NIH AIDS Reagent Program

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HI.fate.SFFV was a gift from Alon Herschhorn & Joseph Sodroski (Addgene plasmid # 191554 ; http://n2t.net/addgene:191554 ; RRID:Addgene_191554)

  • For your References section:

    Intra- and extra-cellular environments contribute to the fate of HIV-1 infection. Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, Achuthan V, Engelman AN, Sodroski J, Herschhorn A. Cell Rep. 2021 Aug 31;36(9):109622. doi: 10.1016/j.celrep.2021.109622. 10.1016/j.celrep.2021.109622 PubMed 34469717
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