MSCV-P C-FlagHA HPV16-18 E7 Chimera C
(Plasmid
#191544)
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PurposeProtein expression of HPV16 E7 Chimera C
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV-P C-FlagHA GAW
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV16-18 E7
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SpeciesVirus
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MutationCR1 HPV18 E7, CR2 C-terminus HPV16 E7
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Tag
/ Fusion Protein
- FlagHA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CR1= 1-15 amino acids of 18E7, CR2-C-terminus = 16-98 of 16E7
Please visit https://www.biorxiv.org/content/10.1101/2022.07.18.500567v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-P C-FlagHA HPV16-18 E7 Chimera C was a gift from Elizabeth White (Addgene plasmid # 191544 ; http://n2t.net/addgene:191544 ; RRID:Addgene_191544) -
For your References section:
ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 Proteins. Nouel J, White EA. mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22. 10.1128/mbio.02033-22 PubMed 36346242