pBabe Smad4J Rescue
(Plasmid #19153)

• Depositing Lab: Joan Massague
• Publication: Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Backbone

• Vector backbone: pBABE-hygro
• Backbone size w/o insert (bp): 5558
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow in STBL3 cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD family member 4
• Alt name: DPC4, JIP, MADH4
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Mutation: G to A at bp 1397 T to C at bp 1398
• Entrez Gene: SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Padua
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Site directed mutagenesis with quickchange kit