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AAVS1_Puro_H2BK_mNG_hSLBP(18-108)
(Plasmid #191529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene plasmid # 68375)
  • Backbone size w/o insert (bp) 5737
  • Total vector size (bp) 7412
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2BK_mNG_hSLBP(18-108)
  • Alt name
    SLBP probe
  • Species
    H. sapiens (human), Synthetic
  • GenBank ID
    7884
  • Tag / Fusion Protein
    • mNeonGreen (mNG) (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-mNG_hSLBP(18-108) expression is driven by the human HIST1H2BK promoter and 3'UTR sequences.

-Use in combination with eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Plasmid #79888)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1_Puro_H2BK_mNG_hSLBP(18-108) was a gift from Yannick Doyon (Addgene plasmid # 191529 ; http://n2t.net/addgene:191529 ; RRID:Addgene_191529)
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