AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
(Plasmid #191527)

• Purpose: Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
• Depositing Lab: Yannick Doyon
• Publication: S-phase specific probe (unpublished)

Backbone

• Vector backbone: AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene plasmid # 68375)
• Backbone size w/o insert (bp): 5737
• Total vector size (bp): 7270
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hGEM(1-110)_mNG_hSLBP(18-108)
• Alt name: S-phase specific probe tagged with 3xFLAG-2xSTREP tag
• Species: H. sapiens (human), Synthetic
• GenBank ID: 51053 7884
• Tag / Fusion Protein:
  • mNeonGreen (mNG) + 3xFLAG_TwinStrep (C terminal on insert)

Resource Information

• Supplemental Documents:
  • AAVS1_Puro_PGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS_pA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

--Expression is driven by the human PGK1 promoter and BGH polyA

-hGEM(1-110) sequence is derived from the original FUCCI system PMID: 18267078

-Use in combination with eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Plasmid #79888)