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Addgene

pRetroSuper shSmad4J hygro
(Plasmid #19151)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroSuper-Hygro
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    small hairpin RNA against SMAD family member 4
  • Alt name
    DPC4, JIP, MADH4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Padua
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

19nt targeting sequence - 5'-GGTGTGCAGTTGGAATGTA- 3' (SMAD4)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroSuper shSmad4J hygro was a gift from Joan Massague (Addgene plasmid # 19151 ; http://n2t.net/addgene:19151 ; RRID:Addgene_19151)

  • For your References section:

    TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J. Cell. 2008 Apr 4. 133(1):66-77. 10.1016/j.cell.2008.01.046 PubMed 18394990
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