pENTR - CaSR-EGFP
(Plasmid
#191503)
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PurposeA zebrafish CaSR-EGFP construct used for Gateway cloning.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
- Backbone size w/o insert (bp) 2656
- Total vector size (bp) 6476
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Modifications to backbonenone
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Vector typezebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaSR-EGFP-SV40
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3810
- Promoter none
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR - CaSR-EGFP was a gift from Michael Granato (Addgene plasmid # 191503 ; http://n2t.net/addgene:191503 ; RRID:Addgene_191503) -
For your References section:
The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons. Shoenhard H, Jain RA, Granato M. Cell Rep. 2022 Dec 6;41(10):111790. doi: 10.1016/j.celrep.2022.111790. 10.1016/j.celrep.2022.111790 PubMed 36476852