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pTol1 - 14xUAS:CaSR-EGFP
(Plasmid #191502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTol1-14XUAS:NTR-TagRFPT
  • Backbone manufacturer
    Harold Burgess lab (NIH)
  • Backbone size w/o insert (bp) 3860
  • Total vector size (bp) 8514
  • Modifications to backbone
    swapped out NTR-TagRFPT to CaSR-EGFP-SV40 using inFusion kit from Takara Biosciences
  • Vector type
    creation of transgenic fish using Tol1 transgenesis method

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CaSR-EGFP-SV40
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3810
  • Promoter 14X UAS

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Backbone was provided by the lab of Dr. Harold Burgess (NIH)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol1 - 14xUAS:CaSR-EGFP was a gift from Michael Granato (Addgene plasmid # 191502 ; http://n2t.net/addgene:191502 ; RRID:Addgene_191502)

  • For your References section:

    The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons. Shoenhard H, Jain RA, Granato M. Cell Rep. 2022 Dec 6;41(10):111790. doi: 10.1016/j.celrep.2022.111790. 10.1016/j.celrep.2022.111790 PubMed 36476852
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