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pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
(Plasmid #191500)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191500 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDesttol2pACrymCherry (Addgene #64023)
  • Backbone manufacturer
    Berger and Currie 2013
  • Backbone size w/o insert (bp) 7480
  • Total vector size (bp) 15712
  • Vector type
    creation of transgenic fish using Tol2 transgenesis method

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CaSR-EGFP-SV40
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3810
  • Promoter myo6b

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    p5e myo6b Gateway Cloning construct was provided by the lab of Dr. Katie Kindt (NIH)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry was a gift from Michael Granato (Addgene plasmid # 191500 ; http://n2t.net/addgene:191500 ; RRID:Addgene_191500)

  • For your References section:

    The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons. Shoenhard H, Jain RA, Granato M. Cell Rep. 2022 Dec 6;41(10):111790. doi: 10.1016/j.celrep.2022.111790. 10.1016/j.celrep.2022.111790 PubMed 36476852
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