Lentiviral-FKBP-cpGFP-Nb39
(Plasmid
#191498)
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PurposeRapamycin-dependent opioid sensor for opioid receptors (component 2)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentiviral
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP-cpGFP-Nb39
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lentiviral-FKBP-cpGFP-Nb39 was a gift from Wenjing Wang (Addgene plasmid # 191498 ; http://n2t.net/addgene:191498 ; RRID:Addgene_191498) -
For your References section:
A Modular Fluorescent Sensor Motif Used to Detect Opioids, Protein-Protein Interactions, and Protease Activity. Kroning KE, Li M, Shen J, Fiel H, Nassar M, Wang W. ACS Chem Biol. 2022 Aug 19;17(8):2212-2220. doi: 10.1021/acschembio.2c00364. Epub 2022 Aug 4. 10.1021/acschembio.2c00364 PubMed 35925780