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Addgene

3'-ecRESCUE-mCherry
(Plasmid #191486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC0078 RESCUE
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 130661)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dRanCas13b-hADAR2-mCherry-ecDHFR
  • Species
    H. sapiens (human); R. anatipestifer, E. coli
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, tmr, tmrA)
  • Entrez Gene
    ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, NEDHYMS, RED1)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3'-ecRESCUE-mCherry was a gift from Xu Ma (Addgene plasmid # 191486 ; http://n2t.net/addgene:191486 ; RRID:Addgene_191486)

  • For your References section:

    ecRESCUE: a novel ecDHFR-regulated RESCUE system with reduced RNA off-targeting activity. Wang Y, Li G, Li X, Wang Y, Huang X, Hu X, Gao J. Cell Commun Signal. 2021 Jul 31;19(1):81. doi: 10.1186/s12964-021-00759-2. 10.1186/s12964-021-00759-2 PubMed 34332602
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