pLV-SARS-CoV-2 SL1 alone-Luciferase
(Plasmid
#191482)
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PurposeLuciferase reporter for SARS-CoV-2 5' UTR SL1 alone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Vector typeLentiviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 5' UTR SL1 alone
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SpeciesSevere acute respiratory syndrome coronavirus 2
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MutationContains only the stem-loop1 region of SARS-CoV-2 5' UTR
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SARS-CoV-2 SL1 alone-Luciferase was a gift from Hao Wu (Addgene plasmid # 191482 ; http://n2t.net/addgene:191482 ; RRID:Addgene_191482) -
For your References section:
SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. Shi M, Wang L, Fontana P, Vora S, Zhang Y, Fu TM, Lieberman J, Wu H. bioRxiv. 2020 Sep 18. doi: 10.1101/2020.09.18.302901. 10.1101/2020.09.18.302901 PubMed 32995777
