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Addgene

pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase
(Plasmid #191481)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 5' UTR ΔSL1
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Mutation
    Missing the stem-loop 1 region of SARS-CoV-2 5' UTR

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase was a gift from Hao Wu (Addgene plasmid # 191481 ; http://n2t.net/addgene:191481 ; RRID:Addgene_191481)

  • For your References section:

    SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism. Shi M, Wang L, Fontana P, Vora S, Zhang Y, Fu TM, Lieberman J, Wu H. bioRxiv. 2020 Sep 18. doi: 10.1101/2020.09.18.302901. 10.1101/2020.09.18.302901 PubMed 32995777
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