pLV-SARS-CoV-2 5'UTR-Luciferase
(Plasmid
#191480)
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PurposeLuciferase reporter for SARS-CoV-2 5' UTR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Vector typeLentiviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 5' UTR
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Mutationcontains SARS-CoV-2 5' UTR
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SARS-CoV-2 5'UTR-Luciferase was a gift from Hao Wu (Addgene plasmid # 191480 ; http://n2t.net/addgene:191480 ; RRID:Addgene_191480) -
For your References section:
Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion. Vora SM, Fontana P, Mao T, Leger V, Zhang Y, Fu TM, Lieberman J, Gehrke L, Shi M, Wang L, Iwasaki A, Wu H. Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2117198119. doi: 10.1073/pnas.2117198119. 10.1073/pnas.2117198119 PubMed 35149555
