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Addgene

pQCXIH BRGI-Flag
(Plasmid #19148)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIH
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
  • Alt name
    SMARCA4, BRG1; SNF2; SWI2; BAF190; SNF2L4; SNF2LB; hSNF2b; FLJ39786; SNF2-BETA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5700
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Qiaoran Xi Human BRGI cDNA (from pFASTBAC-BAF170 Kingston's lab) with Flag tag at c-terminus
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

human BRGI with Flag at C-terminus PCR product cut with SalI then blunted and ligated into blunt BamHI site in vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIH BRGI-Flag was a gift from Joan Massague (Addgene plasmid # 19148 ; http://n2t.net/addgene:19148 ; RRID:Addgene_19148)

  • For your References section:

    Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the transforming growth factor beta transcriptional program. Xi Q, He W, Zhang XH, Le HV, Massague J. J Biol Chem. 2008 Jan 11. 283(2):1146-55. 10.1074/jbc.M707479200 PubMed 18003620
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